CEEX III 227/2006

METODE MODERNE DE DIAGNOSTIC SI CERCETARE, IMUNOHISTOCHIMIE, BIOLOGIE MOLECULARA, CONTROLUL CALITĂŢII
 COLECTIV DE LUCRU
Prof. dr.  Pavai Zoltan
Prof. Dr.  Jung Janos
As. Univ. Horvath Emoke
As. Univ. Pap Zsuzsanna
Prep. Univ. Gurzu Simona
Prof. Dr. Branzaniuc Klara
As. Univ. Denes Lorand
Prep. Univ. Mezei Tibor
Munteanu Paraschiva

Proiectul a prezentat trei obiective majore:
1. Informarea publicului din ţară prin atragerea personalităţilor de remarcă din domeniu din Europa prin organizarea unui congres European, şi verificarea acumulării de cunoştinţe prin Workshop naţional.
2. Acumulare de noi informaţii prin deplasări în străinătate şi realizarea unor conexiuni cu Laboratoare de remarca din străinătate (stagii, participare la congres)
3. Diseminarea cunoştinţelor dobândite, info days, site web, publicaţii

1. Organizarea unei Conferinţe Internaţionale cu tema:,” Metode moderne de diagnostic si cercetare, imunohistochimie, biologie moleculara, controlul calităţii”
In perioada septembrie decembrie 2006 a fost format comitetul de organizare, au fost contactaţi lectorii si trimise primele anunţuri. A fost pregătit situl congresului
Congresul s-a desfăşurat în perioada 12-15 aprilie la Tg.-Mures în sălile Hotelului Continental. Au fost prezenţi 150 participanţi dintre care 130 din ţară, din care 20 asistente de laborator şi 20 participanţi din străinătate. In cadrul conferinţei au fost prezentate lucrări din domeniul imunohistochimiei, biologiei moleculare şi a controlului calităţii pentru medici, s-a organizat secţiune de prezentare (teoretică şi practică) a metodei de imunohistochimie pentru asistente medicale. S-a organizat o masă rotundă pe tema controlului calităţii în laboratoarele de morfopatologie şi aspectele acreditării laboratoarelor.
Ca si rezultate imediate
- Laboratorul de Anatomie patologică a Spitalului Clinic Judeţean a fost acceptat în sistemul de controlul calităţii UK NEQAS. În perioada 2007-2009 pană acum participând la 5 runde.
- S-a iniţiat organizarea unui modul de controlul calităţii al lamelor histologice, care a avut prima întrunire la Conferinţa de Morfologie din Craiova 17-20 mai, rezultatele evaluării secţiunilor de hematoxilina eozina, predate de 15 laboratoare din tara fiind prezentate pe situl www.memodic.ro

Achiziţionarea aparaturii. In cursul programului au fost achiziţionate aparatura de informatică (laptop, printer, scanner) pentru asigurarea comunicarea cu participanţii congresului, si aparatura de cercetare (aparat produs gheaţă, hibridizator HyBrite) pentru dotarea laboratorului de Biologie moleculară a Disciplinei de Anatomie.

2.Organizarea de stagii în domeniul aplicaţiilor imunohistochimiei si altor metode moderne de cercetare si a controlului calităţii
- In perioada 14-28 noiembrie doi doctoranzi au participat la un stagiu de pregătire in imunohistochimie si biologie moleculara la Laboratorul de Patologie din cadrul Institutului Oncologic Naţional din Budapesta
- In perioada iunie 2007 doi doctoranzi si un cercetător cu experienţa au efectuat deplasare la Laboratorul de Biologie Moleculara a institutului de Patologie a Universităţii Jageloniene din Cracovia. In cadrul stagiului s-au însuşit metode de biologie moleculara: extragerea ADN-ului din sânge si material bioptic, amplificarea ADN prin PCR, examinarea produsului prin SSCP, examinarea secvenţei ADN prin utilizarea unui secvenator ABI 700. Rezultatele vor fi publicate de către partenerul polonez, cu menţionarea echipei din România
- In perioada noiembrie 2 cercetători cu experienţa având si calitate de Prorector ştiinţific si Directorul Scolii Doctorale au efectuat o deplasare la Institutul Oncologic din Budapesta pentru stabilirea unor noi proiecte comune in cadrul Scolii Doctorale.
- In perioada 12-30 noiembrie 2 cercetători cu experienţă au efectuat un stagiu de 2 săptămâni la Institutul Karolinska din Stocholm Suedia, la Centrul de Microbiologie si Biologie Tumorala. In cursul acestuia s-a aprofundat utilizarea metodei de FISH (Hibridizare Fluorescenta in Situ) in cazuri de boli mieloproliferative, si s-a conturat o colaborare in domeniul respectiv

3. Participarea la un Congres Internaţional pe tema aplicării metodelor moderne in diagnostic.
În perioada 7-12 octombrie, 2006 2 cercetători au participat la al XIII Meeting of the European Association for Haematopathology din Viena. Curs creditat de EACCME cu 30 puncte, respectiv de Osterreichische Academie der Arzte cu 52 puncte, punctaj recunoscut şi de American Medical Association.
În programul cursului au fost incluse mai multe simpozioane legate de problemele de diagnostic şi diagnostic diferenţial de hematopatologie: EAHP Bone Marrow Symposium, EAHP Bone Marrow Workshop, EAHP Lymphoma Symphosium şi EAHP Lymphoma Workshop, fiind invitaţi 51 lectori experţi în hematopatologie (lista persoanelor invitate pe pagina web. www.mondial.at/eahp).

4. Organizarea unui Workshop naţional pentru analiza aplicării rezoluţiilor congresului
Din cauza întârzierii semnării contractului pe anul 2008 datele limita pentru organizarea evenimentelor iniţial planificate s-au suprapus cu perioada de conferinţe internaţionale si naţionale.
În colaborare cu Şcoala Doctorala a UMF Tg.-Mures in zilele de 8-9 mai 2008. cu  30 de participanţi din tară. s-a organizat mesa rotundă si cursul de perfecţionare  Utilizarea Metodelor imunohistochimice în diagnostic şi cercetare.

5. Diseminarea activităţilor
5.2 Publicarea de articole în reviste de specialitate. Activitate cu caracter permanent

A fost acceptat pentru publicare articolul:
Plasmablastic lymphoma associated with Human Immunodeficiency Virus, Case report. Emőke Horváth 1), László Krenács 2), Enikő Bagdi 2), Zoltán Pávai1), Ioan Macarie3), Előd-Ernő Nagy4), Smaranda Demian3) Romanian Journal of Morphology and Embryology vol. 49 nr. 3/2008;. ISSN 1220-0522 (PubMed)CNCSIS B+
A apărut
Imunohistochimia organului vomeronazal uman L Denes, Zsuzsanna Pap, Z Pavai. Revista Română de Anatomie funcţională şi clinică, macro şi microscopică şi de Antropologie. Vol. VII, Nr2, 2008 pag 178-182 ISSN 1583-4026, CNCSIS B

Rezultatele obţinute in urma congresului şi a deplasărilor au fost prezentate in cadrul
Volumul de rezumate a lucrărilor ca şi supliment la Revista de Medicina si Farmacie UMF Tg.-Mures Vol. 53/2007 supl. 2 ISSN 1221-2229. conţinând 19 lucrări
1st conference in New Perspectives in Cancer Pathology. May 24-27 2007, Bucharest. 1 lucrare
Simpozionului aniversar al Institutului Naţional Victor Babeş The (un)predictibile future of cellular and molecular medicine, şi al 4lea Simpozion naţional de patologie. 31 oct-2 nov Bucureşti. 4 lucrări
Sesiunea de comunicări ale UMF Tg.-Mures in decembrie 2007 2 lucrări
Al IX-lea Congres Naţional al Societăţii Anatomiştilor din România. Iaşi 22-24 mai 4 lucrări
Al IX-lea Congres Naţional al Societăţii Romane de Morfologie Craiova 28-31 Mai 4 lucrări

5.5 S-a creat şi este menţinut Situl www.memodic.ro
Ca urmare a colaborării cu invitaţii din cadrul conferinţei MEMODIC faza a II-a. A fost predat un proiect in cadrul FP7, directorul de proiect având calitate de  Conducătorul partenerului roman..
Call Selected: FP7-HEALTH-2007-B, Sub-Sheme Selected: CP-IP, Proposal ID: 223652,
Title: Standardization of ICC and ISH staining in cancer diagnostic across Europe trough a centralised EQA program, Coordinator: Karl-Johan Pluzek
În luna aprilie 2008 a fost anunţat că proiectul Nu a fost admis pentru finanţare.

Am finalizat contractul de Sponsorizare pentru cercetare pentru Proiectul: Standardizarea si implementarea tehnicilor de monitorizare prin RT-PCR a pacienţilor cu Leucemie Mieloida Cronica in cadrul Laboratorului de Biologie Moleculara a UMF TG.-Mures. cu Firma Bristol Myers Squib. In Cadrul acestuia in colaborare cu Clinica de Hematologie si Transplant vom efectua investigaţii de biologie moleculara in tema Leucemiilor Mieloide Cronice Nr. Contract UMF 3773/25.04.2008.

Bazat pe colaborarea cu Institutul Karolinska si Universitatea Jageloniana s-a depus la CNCSIS Idei proiect cu nr. înregistrare 1729:  Evidenţierea unor potenţiali factori responsabili pentru transformarea leucemiei mieloide cronice in faza accelerata sau blastica prin metoda multipoint fish a cromozomului 3

CEEX III 227/2006

Modern methods in diagnosis and research, immunohistochemistry, molecular biology, quality control
PROJECT TEAM
Prof. dr  Pavai Zoltan
Prof. Dr.  Jung Janos
As. Univ Horvath Emoke
As. Univ. Pap Zsuzsanna
Prep. Univ. Gurzu Simona
Prof. Dr. Branzaniuc Klara
As. Univ. Denes Lorand
Prep. Univ. Mezei Tibor
Munteanu Paraschiva

The project had three major objectives:
1. Information dissemination for the Romanian researchers by inviting renowned European personalities working in the field, by organizing an European symposium and evaluation of knowledge assimilation through a national Workshop.
2. Knowledge assimilation through several research trips abroad and establishment of new working relationships with several high class research laboratories abroad (research stays, congresses).
3. Distribution of the acquired information, info days, website, publications.
1. Organizing of an International Symposium named: “Modern methods in diagnosis and research, immunohistochemistry, molecular biology, quality control”.
During the September – December 2006 period the organizing committee had been established, the speakers had been contacted and the first announcements had been dispatched. The website of the symposium had been set up.
The Symposium took place between 12-15 April, in Tg.-Mures, at the Continental Hotel. There were 150 attendants, of which 130 from Romania (including 20 laboratory assistants) and 20 people from abroad. During the Symposium, in the section for doctors, there were presented studies from the field of immunohistochemistry, molecular biology and quality control. In the section for laboratory assistants we organized a presentation (theoretical and practical) of the immunohistochemistry method. These were followed by a round table dealing with quality control of the pathology laboratories and different aspects of their authorization.
Immediate results:
- The Pathology Department of the Tg.-Mures Clinical County Hospital has been accepted in the British quality control system UK NEQAS. In the 2007-2009 timeframe, until now it took part in 5 rounds.
- We initiated the establishment of a histological slide quality control module, that had it’s first meeting at the Morphology Symposium in Craiova, between 17-20 of May. The evaluation results of hematoxilin-eosin stained slides, handed in by 15 laboratories from Romania, are presented on the www.memodic.ro website.

Purchase of the equipment: During the project we purchased computer equipment (laptop, printer, scanner) in order to ensure communication with the participants and also research equipment (ice making machine, HyBrite hybridizer) for the Molecular biology laboratory of the Department of Anatomy.

2. Research trips in the fields of applied immunohistochemistry and other modern research methods, and quality control
- between 14-28 November two PhD students were on a training course in immunohistochemistry and molecular biology at the Pathology Laboratory of the National Oncology Institute in Budapest.
- in June 2007 two PhD students and a senior researcher were on a research trip to the Molecular Biology Laboratory of the Jagellonian University Pathology Institute in Krakow. During their stay they were trained in the use of several molecular biology methods: DNA extraction from blood and biopsy material, DNA amplification by PCR, product evaluation using SSCP, DNA sequencing with an ABI 700 sequencer. The results will be published by the polish partner, mentioning the Romanian team.
- In November 2007 two senior researchers, the Scientific Vice-rector and the Head of the PhD School visited the Oncology Institute in Budapest, in order to initiate new joint projects for the PhD School.
- between 12-30 November 2007 two senior researchers were on a 2 week research trip to the Microbiology and Tumorbiology Center of the Karolinska Institute in Stockholm, Sweden. During their stay they perfected their knowledge of the FISH method (In Situ Fluorescent Hybridization) in myeloproliferative diseases and laid the foundations of a new partnership in this field.

3. Attending an International Congress on the application of modern methods in diagnosis
Between 7-12 October 2006, two researchers attended the XIIIth Meeting of the European Association for Haematopathology in Vienna. This course was credited with 30 points by the EACCME, and 52 points by the Osterreichische Academie der Arzt, these certifications being recognized also by the American Medical Association.
The program of the course included several symposia on hematopathological diagnostic and differential diagnosis problems: EAHP Bone Marrow Symposium, EAHP Bone Marrow Workshop, EAHP Lymphoma Symposium and EAHP Lymphoma Workshop. There were 51 invited lecturers, haematopathology experts (the full list can be found at: www.mondial.at/eahp).

4. Organizing a national Workshop in order to evaluate the application of the decisions taken at the Symposium
Due to the delay in the signing of the contract for 2008, the deadlines for organizing previously planned events overlapped the time period of the national and international congresses.
In collaboration with the PhD School of UMF Tg.-Mures, on 8-9 May 2008 we organized a round table and training course, attended by 30 people, entitled: The use of immunohistochemical methods in research and diagnosis.

5. Dissemination of the activities
5.2 Publishing in specialized journals. Ongoing activity
The following paper has been accepted for publishing:
Plasmablastic lymphoma associated with Human Immunodeficiency Virus, Case report. Emőke Horváth 1), László Krenács 2), Enikő Bagdi 2), Zoltán Pávai1), Ioan Macarie3), Előd-Ernő Nagy4), Smaranda Demian3) Romanian Journal of Morphology and Embryology vol 49 nr 3/2008; ISSN 1220-0522 (PubMed)CNCSIS B+
Published article:
Immunohistochemistry of the human vomeronasal organ; L Denes, Zsuzsanna Pap, Z Pavai; Revista Română de Anatomie funcţională şi clinică, macro şi microscopică şi de Antropologie. Vol VII, Nr2, 2008 pag 178-182 ISSN 1583-4026, CNCSIS B

The results of the congress and research and training trips were presented as follows:
Abstracts book, appendix of the Revista de Medicina si Farmacie UMF Tg.Mures Vol 53/2007 supl. 2 ISSN 1221-2229., containing 19 papers
1st confrerence in New Perspectives in Cancer Pathology. May 24-27 2007, Bucharest. 1 paper
Anniversary Symposium of the Victor Babes National Institute - The (un)predictable future of cellular and molecular medicine, and the 4th National Symposium of Pathology, 31 Oct-2 Nov Bucharest. 4 papers
Symposium of the UMF Tg.-Mures in December 2007 2 papers
The IXth National Congress of the Anatomical Society of Romania, Iasi, 22-24 May 4 papers
The IXth National Congress of the Romanian Society of Morphology, Craiova 28-31 May 4 papers

5.5 Setting up and maintaining of the www.memodic.ro website.
As a result of the collaboration with the invited lecturers of the MEMODIC symposium a project has been set up as part of the FP7 framework, the head of the MEMODIC project being assigned head of the Romanian partner.
Call Selected: FP7-HEALTH-2007-B, Sub-Scheme Selected: CP-IP, Proposal ID: 223652,
Title: Standardization of ICC and ISH staining in cancer diagnostic across Europe trough a centralized EQA program, Coordinator: Karl-Johan Pluzek
In April 2008 it has been announced that the project has NOT been accepted for financing.

We finalized the Research sponsorship contract with Bristol Myers Squibb, for the project: Standardizing and implementing RT-PCR monitoring techniques of patients with chronic myeloid leukemia at the Molecular Biology Laboratory of UMF Tg.-Mures. In collaboration with the Hematology and Transplant Clinic we will perform molecular biology studies on chronic myeloid leukemias, contract no: UMF 3773/25.04.2008.

Based on the collaboration with the Karolinska Institute and Jagellonian University we wrote a project plan, sent to CNCSIS Idei proiect, registration no: 1729: Identification of potential factors responsible for chronic myeloid leukemia transformation into accelerated or blastic phase, using the multipoint fish method of the 3rd chromosome.